Estefanía Enebral-Romero, Daniel García-Fernández, Laura Gutiérrez-Gálvez, David López-Diego, Mónica Luna, Adrián García-Martín, Elena Salagre, Enrique G. Michel, Íñigo Torres, Félix Zamora, Tania García-Mendiola, Encarnación Lorenzo, Bismuthene – Tetrahedral DNA nanobioconjugate for virus detection,
Biosensors and Bioelectronics, Volume 261, 2024, 116500, ISSN 0956-5663, https://doi.org/10.1016/j.bios.2024.116500.
In this work, we present an electrochemical sensor for fast, low-cost, and easy detection of the SARS-CoV-2 spike protein in infected patients. The sensor is based on a selected combination of nanomaterials with a specific purpose. A bioconjugate formed by Few-layer bismuthene nanosheets (FLB) and tetrahedral DNA nanostructures (TDNs) is immobilized on Carbon Screen-Printed Electrodes (CSPE). The TDNs contain on the top vertex an aptamer that specifically binds to the SARS-CoV-2 spike protein, and a thiol group at the three basal vertices to anchor to the FLB. The TDNs are also marked with a redox indicator, Azure A (AA), which allows the direct detection of SARS-CoV-2 spike protein through changes in the current intensity of its electrolysis before and after the biorecognition reaction. The developed sensor can detect SARS-CoV-2 spike protein with a detection limit of 1.74 fg mL−1 directly in nasopharyngeal swab human samples. Therefore, this study offers a new strategy for rapid virus detection since it is versatile enough for different viruses and pathogens.
Keywords
Nanostructured bioconjugate; Tetrahedral DNA nanostructure (TDN); SARS-CoV-2 aptamer-based tetrahedral DNA nanostructure (TDNapt); Aptasensor; Bismuthene nanosheets
Full Text Link:
https://www.sciencedirect.com/science/article/pii/S0956566324005050